T4 Triiodothyronine ELISA TEST KIT

INTENDED USE

Immunoassay for the in vitro quantitative determination of thyroxine in human serum.

Materials provided

• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with T4 derivant.

• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 2.5(B), 5(C), 10(D), 15(E) and 30(F) μg/dL.

• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase) labeled mouse monoclonal T4 in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.2% ProClin300 preservative. ANS 1.5 mg/mL.

• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.

• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.

• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated), PBS-Tween wash solution.

• IFU, 1 copy.

• Plate Lid: 1 piece.

Materials required (but not provided)

• Microplate reader with 450nm and 620nm wavelength absorbent capability.

• Microplate washer.

• Incubator.

• Plate shaker.

• Micropipettes and multichannel micropipettes delivering 50μl with a precision of better than 1.5%.

• Absorbent paper.

• Distilled water

Storage

• Store at 2-8℃.

• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date, whichever is earlier.

• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at -20 °C. Avoid multiple freeze-thaw cycles.

• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.

Laboratory T4 Triiodothyronine ELISA TEST KIT

T4 Triiodothyronine ELISA TEST KIT

Hospital T4 Triiodothyronine ELISA TEST KIT

Commonly used thyroid hormone test T4 Triiodothyronine ELISA TEST KIT Images